This video describes the surgical procedure to immobilize the laser Doppler probe over the murine brain's middle cerebral artery, or MCA. The probe helps to measure the blood flow in the brain's microvasculature by measuring the Doppler shift caused due to the motion of red blood cells within the artery.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Preparation and positioning of the laser Doppler Prepare a holder for the laser Doppler probe by cutting the tip of a 10 µL pipet tip (3-5 mm length). NOTE: All instruments are sterilized using a hot bead sterilizer. Surfaces are also disinfected before and after surgery wit…
Positioning Laser Doppler Probe: A Technique to Implant Probe Over Middle Cerebral Artery for Blood Flow Measurement in Murine Model. J. Vis. Exp. (Pending Publication), e20806, doi: (2023).