Porcine Heart Excision: A Protocol to Extract Porcine Heart for Cardiac Transplant
Published: April 30, 2023
Abstract
Source: Hatami, S., et al. Normothermic Ex Situ Heart Perfusion in Working Mode: Assessment of Cardiac Function and Metabolism. J. Vis. Exp. (2019).
In this video, we describe a step-by-step protocol to extract the heart from a porcine model for cardiac transplantation.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Blood Collection and Heart Procurement Evaluate the anesthesia level every minimum of 5 min to confirm the surgical plane (no pedal reflex and no blink reflex, no response to painful stimuli). Perform a median sternotomy. Identify jugulum and xiphoid as landmarks. Using electrocautery, d…
Divulgazioni
The authors have nothing to disclose.
Materials
| Debakey-Metzenbaum dissecting scissors | Pilling | 342202 | |
| MAYO dissecting scissors | Pilling | 460420 | |
| THUMB forceps | Pilling | 465165 | |
| Debakey straight vascular tissue forceps | Pilling | 351808 | |
| CUSHING Gutschdressing forceps | Pilling | 466200 | |
| JOHNSON needle holder | Pilling | 510312 | |
| DERF needle holder | Pilling | 443120 | |
| Sternal saw | Stryker | 6207 | |
| Sternal retractor | Pilling | 341162 | |
| Vorse tubing clamp | Pilling | 351377 | |
| MORRIS ascending aorta clamp | Pilling | 353617 | |
| Surgical snare (tourniquet) set | Medtronic | CVR79013 | |
| 2-0 SILK black 12" x 18" strands | ETHICON | A185H | |
| 3-0 PROLENE blue 18" PS-2 cutting | ETHICON | 8687H | |
| DLP cardioplegia cannula | Medtronics | 20613994495406 | Aortic root cannula |
| 5F Ventriculr straight pigtail cathter | CORDIS | 534550S | |
| 5F AVANTI+ Sheath Introducer | CORDIS | 504605A | |
| Surgical electrocautery device Kls | Martin | ME411 |
Tags
Citazione di questo articolo
Porcine Heart Excision: A Protocol to Extract Porcine Heart for Cardiac Transplant. J. Vis. Exp. (Pending Publication), e20838, doi: (2023).