In this video, we describe a protocol for the encapsulation of porcine oocytes in hydrophobic microbioreactors.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Encapsulation in super-hydrophobic fluorinated ethylene propylene (FEP) microbioreactors. NOTE: Make sure that all in vitro maturation (IVM) procedures are carried out on a thermostatically controlled table, and cumulus-oocyte complexes (COCs) are maintained at 38 °C throughout their handling. <ol…
Liquid Marble Microbioreactor System: A 3D Culture Protocol for Encapsulation of Porcine Oocytes. J. Vis. Exp. (Pending Publication), e20844, doi: (2023).