In this video, we show a procedure to induce aqueous-deficient dry eye disease in a rabbit model by injecting the palpebral portion of the superior lacrimal gland with Concanavalin A. This model is useful for pharmacokinetic and biodistribution assays of drugs for treating dry eye disease.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Animals and housing Acquire New Zealand White (NZW) rabbits weighing 2-3 kg. House the rabbits singly in cages with strict temperature (65 ± 5 °F) and humidity (45 ± 5%) control. Lighting should have a 12 h on/off cycle. Provide unlimited access to water and standard rabbit chow. Eliminate dietary enri…
Representative Results
Figure 1: Gas mask sedation. This photograph shows the gas mask providing brief moderate sedation with isoflurane. Figure 2: Preparation of rabbit for concanavalin A injections. (A) Small shears are used to remove fur, allowing…
Divulgazioni
The authors have nothing to disclose.
Materials
27 gauge needles (5/8)
Becton Dickinson and Company, Franklin Lakes, NJ
305921
Needles for injecting ConA into the lacrimal glands
Aceproinj (acepromazine)
Henry Schein Animal Health, Dublin, OH
NDC11695-0079-8
0.1ml/kg subcutaneously injection for rabbit sedation
Anesthesia vaporizer
VetEquip, Pleasanton, CA
Item #911103
Bishop Harmon Forceps
Bausch and Lomb (Storz), Bridgewater, NJ
E1500-C
Tissue forceps
Concanavalin A
Sigma, St. Louis, MO
C2010
Make 5mg/ml in PBS for injection into rabbit lacrimal glands
fluorescein
AKRON, Lake Forest, IL
NDC17478-253
Dilute to 0.2% with PBS to measure TBUT
Isoflurane
Henry Schein, Melville, NY
29405
lidocaine
Sigma, St. Louis, MO
L5647
1% in PBS for anesthesia agent
PBS (phosphate buffered saline)
Mediatech, Inc. Manassas, VA
21-031-CV
Rabbit, New Zealand White or Dutch Belted (as described in text)
Charles River Labs, Waltham, MA
2-3 kg
Research animals
Surgical Loupes +1.50
Designs for Vision, Bohemia, NY
Specialty item
Provide magnificantion of ocular surface while observing tear break up and performing Concanavalin A injections.
Performing Injection into Palpebral Portion of the Superior Lacrimal Gland: A Technique for Inducing Dry Eye Disease in Rabbit Model. J. Vis. Exp. (Pending Publication), e20909, doi: (2023).