An Experimental Model of Peripheral Ischemia: A Procedure to Generate Peripheral Ischemia via Femoral Artery Ligation in a Rabbit
Published: April 30, 2023
Abstract
Source: Sligar, A. D., et al. Preclinical Model of Hind Limb Ischemia in Diabetic Rabbits. J. Vis. Exp. (2019).
In this video, we demonstrate a surgical procedure to induce peripheral ischemia via femoral artery ligation in a rabbit. This method provides an effective experimental model for peripheral vascular disease research.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Isolation of the femoral artery Anesthetize a New Zealand rabbit (4–6 months old) with pre-induced diabetes and hyperlipidemia, using 20–40 mg/kg ketamine and 2 mg/kg midazolam via subcutaneous injection. Place the rabbit on 1.5%–3% isoflurane (typically 2%) throughout the initial sedation using a mask. Make …
Representative Results
Figure 1: Induction of hind limb ischemia in rabbits through femoral artery ligation and excision. (A) Illustration of the vascular anatomy of the rabbit hind limb. Place ties at all the points marked to ligate the arteries. (B) Surgical field showing the cut down to the femoral artery prior to ligation. (C) Femoral arteries with ligations in place to induce hind limb ischemia.
Divulgazioni
The authors have nothing to disclose.
Materials
| 0.9% Sodium Chloride | Henry Schein Medical | 1537468 / 1531434 | 250 mL bag / 1000 mL irrigation btl |
| 10 mL Syringe | VWR | BD309695 | |
| 27G Needle | VWR | 89219-288 | |
| Acepromazine | Patterson Veterinary | VEDC207 | |
| Alfaxalone | Patterson Veterinary | 07-891-6051 | |
| Buprenorphine SR | ZooPharm | ||
| Hair Clippers | Amazon | B000CQZI3Q | Oster #40 blade |
| Heating Pad | Cincinnati Subzero | 273 | |
| Heating Pad Pump | Gaymar | Gaymar T/Pump | |
| Hemostat | Fine Science Tools | 13009-12 | Curved Mosquito Hemostat |
| Isoflurane | Henry Schein Medical | 1100734 | |
| Ketamine | Patterson Veterinary | 856440301 | |
| Lidocaine | McKesson Medical-Surgical | 239936 | |
| Lidocaine/Prilocaine cream | McKesson Medical-Surgical | 761240 | |
| Metzenbaum Scissors | Fine Science Tools | 14019-13 | |
| Midazolam | Henry Schein Medical | 1215470 | |
| Scalpel | Fine Science Tools | 10003-12 | |
| Scalpel Blade | Fine Science Tools | 10015-00 | #15 blade |
| Silk Sutures | Ethicon-Johnson & Johnson | A183H | 4-0 silk ties 18" |
| Surgical Drapes | Gepco | 8204S | |
| Weitlaner Retractor | Fine Science Tools | 17012-13 |
Tags
Citazione di questo articolo
An Experimental Model of Peripheral Ischemia: A Procedure to Generate Peripheral Ischemia via Femoral Artery Ligation in a Rabbit. J. Vis. Exp. (Pending Publication), e20928, doi: (2023).