This video describes the technique of surgically inducing bacterial infection in the bone marrow of a rabbit by using bone wax to avoid the leakage of bacteria. This rabbit model can be used to study bone infection treatment and bone regeneration.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Preparation of Bone Infection Models Keep male New Zealand white rabbits, aged 3 months, in individual cages, under air-controlled conditions (20 ± 1 °C) and 12 h/12 h light-dark illumination cycles. Offer routine diet and tap water daily. Ensure that at the time of surgery that the rabbit weighs mor…
Representative Results
Figure 1. Surgical preparation of the bone infection model. (A) Shows the punching position on the tibia. The distance between the drilling hole position for injection with S. aureus to the upper end of tibia is 1.5 cm. (B) Incision made in the skin to expose the periosteum. (C) Shows incision made through the periosteum t…
Divulgazioni
The authors have nothing to disclose.
Materials
absorbable surgical suture
Jinghuan
18S0604A
asepsis injector
Jinglong
20170501
bone wax
ETHICON
JH5CQLM
Electric bone drill unit
Bao Kang
BKZ-1
Electric shaver
Codos
3800
New Zealand white rabbits
Zhejiang Experimental Animal Center
SCXK 2014-0047
No. 11 scalpel
Yuanlikang
20170604
pentobarbital sodium
Merk
2070124
S. aureus freeze drying powder
China General Microbiological Culture Collection Center
Generating Rabbit Model of Bone Infection: A Technique of Inducing Chronic Infection by Injecting Bacteria Directly in Bone Marrow. J. Vis. Exp. (Pending Publication), e20934, doi: (2023).