Matrigel Growth Factor Reduced (GFR) Basement Membrane Matrix, *LDEV-Free, 10 ml |
Corning |
354230 |
Store at -20 °C. |
DMEM/F-12 |
Life Technologies |
11320-033 |
Store at 4 °C. |
Costar 6 Well Clear TC-Treated Multiple Well Plates |
Corning |
3506 |
|
Essential 8 Medium |
Life Technologies |
A1517001 |
Store basal medium at 4 °C. Store supplement at -20 °C. |
Y-27632 dihydrochloride |
Tocris |
1254 |
Store at room temp. Once dissolved in H2O, store at -20 °C. |
Sodium Chloride |
Sigma |
S5886-500G |
|
UltraPure 0.5 M EDTA, pH 8.0 |
Life Technologies |
15575-020 |
|
DPBS, no calcium, no magnesium |
Life Technologies |
14190-250 |
|
100 mm TC-Treated Culture Dish |
Corning |
430167 |
|
DR4 MEF 2M IRR – Academic |
GlobalStem |
GSC-6204G |
Store in liquid Nitrogen. |
DMEM, high glucose, pyruvate |
Life Technologies |
11995-040 |
Store at 4 °C. |
Defined Fetal Bovine Serum, US Origin |
HyClone |
SH30070.03 |
Store at -20 °C. Thaw at 4 °C overnight and aliquot. Store aliquots at -20 °C until needed. |
MEM Non-Essential Amino Acids Solution (100X) |
Life Technologies |
11140-050 |
Store at 4 °C. |
4D-Nucleofector Core unit |
Lonza |
AAF-1001B |
Part of the electroporation system. |
4D-Nucleofector X unit |
Lonza |
AAF-1001X |
Part of the electroporation system. |
P3 Primary Cell 4D-Nucleofector X Kit L (24 RCT) |
Lonza |
V4XP-3024 |
Upon arrival, remove Primary Cell Solution and supplement and store at 4 °C. |
StemPro Accutase Cell Dissociation Reagent |
Life Technologies |
A1110501 |
Store at -20 °C. Thaw overnight at 4°C and warm an aliquot in a 37 °C water bath before use. |
NutriStem XF/FF Culture Medium |
Stemgent |
01-2005 |
Store at -20 °C. Thaw overnight at 4 °C |
AAVS1 TALENs (pZT-AAVS1-L1 and pZT-AAVS1-R1) |
Addgene |
52637 and 52638 |
|
|
|
|
|
AAVS1-CAG-EGFP Homologous Recombination donor |
Addgene |
22212 |
|
Puromycin Dihydrochloride |
Life Technologies |
A11138-03 |
Store at -20 °C. Prepare working aliquots of 1 mg/ml in ddH2O. |
Disposable Borosilicate Glass Pasteur Pipettes |
Fisher Scientific |
13-678-20A |
|
Sorvall Legend XTR (Refrigerated), 120 V 60 Hz |
Thermo Scientific |
75-004-521 |
|
TX-750 4 × 750 ml Swinging Bucket Rotor |
Thermo Scientific |
75003607 |
|
Trypan Blue Solution, 0.4% |
Life Technologies |
15250-061 |
|