Oral Intubation of Adult Zebrafish to Administer Experimental Compounds

Published: October 31, 2023

Abstract

Source: Ji, J., Thwaite, R. et al. Oral Intubation of Adult Zebrafish: A Model for Evaluating Intestinal Uptake of Bioactive Compounds. J. Vis. Exp. (2018).

This video demonstrates a technique for orally intubating adult zebrafish to administer experimental compounds into the esophagus and monitor their intestinal uptake. This method offers a valuable model for studying the dynamics of oral prophylactics or other compounds within the intestine.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board 1. Solutions Required Prepare 150 mg/L (for anesthesia) or 300 mg/L (for euthanasia) of ethyl 3-aminobenzoate methanesulfonate (MS-222) solution with water from the aquarium where the zebrafish are maintained. Fill a small tank with 1 L of anesthetic solution and keep it aerated. Fill another small tank with 1 L o…

Divulgazioni

The authors have nothing to disclose.

Materials

Silicone tube Dow Corning 508-001 0.30 mm inner diameter and 0.64 mm outer diameter
Luer lock needle Hamilton 7750-22 31 G, Kel-F Hub
Luer lock syringe Hamilton 81020/01 100 μL, Kel-F Hub
Filtered pipette tip Nerbe Plus 07-613-8300 10 μL
MS-222 Sigma Aldrich E10521 powder
10x PBS Sigma Aldrich P5493
Filter paper Filter-Lab RM14034252
Atto-488 NHS ester Sigma-Aldrich 41698
DMEM Gibco 31966 Dulbecco's modified eagle medium
Maxwell RSC simplyRNA Tissue Kit Promega AS1340

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Citazione di questo articolo
Oral Intubation of Adult Zebrafish to Administer Experimental Compounds. J. Vis. Exp. (Pending Publication), e21757, doi: (2023).

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