This video demonstrates a technique to quantify the relative ratio of macrophages among the total infiltrated immune cell populations in temporal artery biopsy. The immunohistochemical staining of a tissue section affected by autoimmune disease selectively stains the immune cells with specific receptors to visually differentiate them from the rest of the cells.
Protocol
All procedures involving sample collection have been performed in accordance with the institute's IRB guidelines. 1. Histopathological Preparation, and Processing After Obtaining Temporal Artery Biopsy NOTE: The temporal artery biopsy (TAB) is performed under local anesthesia and sterile conditions by a certified surgeon. After surgically obtaining a 3 cm arterial section on the more affected side, the specimen is fixed in fo…
Divulgazioni
The authors have nothing to disclose.
Materials
Microtome
Reichert-Jung
Plain and frosted microscope slides and cover slips
Immunohistochemistry to Study the Relationship Between Macrophages and Infiltrated Immune Cells. J. Vis. Exp. (Pending Publication), e21983, doi: (2024).