Evaluating the Effects of Fecal Microbiota Transplantation in an Interleukin-10 Deficient Mouse

Abstract

Source: Xiao, Y. et al., Therapeutic Evaluation of Fecal Microbiota Transplantation in an Interleukin 10-Deficient Mouse Model. J. Vis. Exp. (2022)

This video demonstrates a procedure of fecal microbiota transplantation in an interleukin-10 deficient mouse with colon inflammation. Colonized fecal microbes in the gut release secondary metabolites, eliciting anti-inflammatory cytokine release and reducing colon inflammation.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Collection of fresh fecal pellets Prepare sterile paper towels, blunt-end forceps, and 50 mL conical tubes. Place some paper towels and forceps in separate autoclave bags and autoclave them at 180 °C in dry heat for 30 min. Use sterile conical tubes as well. Weigh the conical tubes and write down their …

Materials

BD Syringe, 1 mL	Fisher Scientific	14-829-10F
Blunt end forceps	Knipex	926443
C57BL/6J mice	Jackson Lab	664
Centrifuge	Eppendorf	5415R
Conical tubes	ThermoFisher	339650
Curved feeding Needles	Kent Scientific	FNC-20-1.5-2
Glycerol	MilliporeSigma	G5516
IL-10 knockout mice	Jackson Lab	4366
USP normal saline	Grainger	6280
Vaporizer	Euthanex Corp.	EZ-108SA