A Platelet-Rich Plasma Injection into the Sectioned Achilles Tendon of a Rat

Published: May 31, 2024

Abstract

Source: Greimers, L., et al. Effects of Allogeneic Platelet-Rich Plasma (PRP) on the Healing Process of Sectioned Achilles Tendons of Rats: A Methodological Description. J. Vis. Exp. (2018).

This video demonstrates a technique for injecting platelet-rich plasma (PRP) into the sectioned Achilles tendon of rats. A small segment is excised from the Achilles tendon of a recipient rat, the site is sutured, and the rat is allowed to recover. Concurrently, whole blood is collected from a donor rat and centrifuged to obtain PRP, which is then activated and injected into the sutured site of the recipient rat to induce tendon repair.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Animal Preparation Use 132 2-month-old male Sprague-Dawley rats weighing 320-450 g (120 rats for experimentation and 12 rats for blood sampling, Figure 1). Fifteen rats for each group was enough for a power of 0.8 (80% chance of finding statistical significance if the specified effect exists). <li…

Representative Results

Figure 1: Experimental design of the study. Figure 2: Surgical Procedure. (a) The tendon complex after removal of the surrounding fascia. (b) Removal of the planta…

Divulgazioni

The authors have nothing to disclose.

Materials

Xylazine (Xyl-M) VMD none anesthetic
Ketamin (Jétamine 1000 CEVA) CEVA Santé Animale none anesthetic
Buprenorphin (Vetergésic Multidosis) ALSTOE none Painkiller
iso-Betadine MEDA-Pharma none Desinfectant
Resorbable yarn Vicryl 6/0 Johnson & Johnson
Nembutal CEVA Santé Animale none Anesthetic
Paraformaldehyde Sigma-Aldrich P6148 Preserves structure of the tissue
Isopropanol 100% VWR 2,09,22,364
Ethanol 95% VWR 2,08,23,362
Xylene VWR 28973.363
Paraffin VWR LEIC3950.1006
Hematoxylin Millipore 1.15938.0025 Colorant
Eosin Millipore 1.15935.0100 Colorant
Eukitt Sigma-Aldrich 3989 Mounting Medium
CaCl2

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Citazione di questo articolo
A Platelet-Rich Plasma Injection into the Sectioned Achilles Tendon of a Rat. J. Vis. Exp. (Pending Publication), e22265, doi: (2024).

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