MRI引导下的破坏血脑屏障在大鼠模型经颅聚焦超声
Summary
微泡介导的重点超声破坏血脑屏障（BBB）的是一个很有前途的技术，针对大脑中的非侵入性给药<sup> 1-3</sup>。该协议概述MRI引导下经颅血脑屏障破坏大鼠模型的实验过程。
Abstract
聚焦超声（FUS）破坏的血脑屏障（BBB）的是一个日益调查的技术绕过血脑屏障1-5。血脑屏障脑部疾病的药物治疗是一个重大的障碍，因为它限制从血管进入脑组织分子小于约500大小6大通过分子。引起血脑屏障破坏的FUS（BBBD）是暂时的和可逆的4对化学品的优势意味着，诱导高度本地化BBBD的。的FUS的诱导BBBD提供的治疗药物对大脑，组织足够的浓度，否则不会交付广泛的影响调查的一种手段。而广泛的超声参数已被证明成功的破坏血脑屏障2,5,7，在实验过程中有几个关键的步骤，以确保准确定位成功中断。这protocOL轮廓如何实现诱导的大鼠模型BBBD MRI引导下聚焦超音波，一个关键的动物实验的准备和气泡的处理步骤的重点。
Protocol
1。超声和MRI设置在这项研究中使用了核磁共振兼容的三轴聚焦超声系统（聚焦超音波仪器公司，多伦多，安大略，加拿大）。使用两种不同的超声换能器：内部构造551.5千赫球为中心的传感器（曲率半径为60毫米，外直径为75毫米，内直径为20毫米），和1.503兆赫，8部门阵列综合作为一个单一的元素球聚焦换能器（FN = 0.8，光圈= 10厘米）驱动压电陶瓷水听器（Imasonic公司，Vo…
Discussion
动物和微泡的制备此过程中最关键的环节。必须完全去除头发上的动物的头，以避免衰减的超声波束。血脑屏障可以isofluorane麻醉下打乱，但是，它变得更加难以实现一致的开幕式。
应始终与保健和小计处理的微气泡，大口径的针头必须使用绘图时，为了避免打破他们。同样，可以合理地用在尾静脉最小的压力表导管应采用建议（22号）。如果需要一个较小的导管，以实现妥?…
Divulgazioni
The authors have nothing to disclose.
Acknowledgements
笔者想感谢他们的帮助与照顾动物肖纳赖德奥特格罗斯和亚历山德拉Garces，平吴为她的技术援助。由国立卫生研究院下，没有为这项工作提供了支持。 EB003268，加拿大研究主席的计划。
Materials
| Name of the reagent | Company | Catalogue number |
| Small Animal Focused Ultrasound System | FUS Instruments, Inc. | RK-100 |
| Definity | Lantheus Medical Imaging | |
| Omniscan | GE Healthcare |
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Tags
MRI-guidedDisruptionBlood-brain BarrierTranscranial Focused UltrasoundRat ModelFUSPharmaceutical TreatmentsBrain DisordersMoleculesVasculatureSizeTemporaryReversibleChemical MeansLocalizedTherapeutic AgentsConcentrationUltrasound ParametersExperimental ProcedureAccurate TargetingAnimal PreparationMicrobubble Handling
Citazione di questo articolo
O’Reilly, M. A., Waspe, A. C., Chopra, R., Hynynen, K. MRI-guided Disruption of the Blood-brain Barrier using Transcranial Focused Ultrasound in a Rat Model. J. Vis. Exp. (61), e3555, doi:10.3791/3555 (2012).