Erratum: Visualizing RNA Localization in Xenopus Oocytes
Published: June 10, 2011
Summary
A correction to the step 2 of Part 1 for article Visualizing RNA Localization in Xenopus Oocytes.
Protocol
A correction was made to Visualizing RNA Localization in Xenopus Oocytes. There was an error in step 2 of part 1. Some characters in the reagents table had the incorrect symbols. This was corrected to: a. 10X Tx buffer (see M&M)2 μl b. 20X cap/NTP mix (see M&M)1 μl c. 1 mM Alexa Fluor 546-14-UTP (Invitrogen)1 μl d. UTP, [α-32P](1 μCi/μl) (Perkin Elmer)1 μl e. DEPC-H2O11 μl f. 0.2 M DTT1 μl g. RNasin (Promega)1 μl h. linear template DNA1 μl i. RNA Polymerase (Promega)1 μl instead of: a. 10′ Tx buffer (see M&M)2 μl 20′ cap/NTP mix (see M&M)1 μl 1 mM Alexa Fluor 546-14-UTP (Invitrogen)1 μl UTP, [α-32P](1 μCi/μl) (Perkin Elmer)1 μl a. DEPC-H2O11 μl b. 0.2 M DTT1 μl c. RNasin (Promega)1 μl d. linear template DNA1 μl e. RNA Polymerase (Promega)1 μl
