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In vitro Organoid Kultur i Primary Mouse tyktarmstumorer
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JoVE Journal Medicine
In vitro Organoid Culture of Primary Mouse Colon Tumors

In vitro Organoid Kultur i Primary Mouse tyktarmstumorer

DOI:
10.3791/50210-v

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07:33 min

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May 17, 2013

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,
1Department of Molecular & Integrative Physiology,University of Michigan , 2Department of Internal Medicine, Division of Gastroenterology,University of Michigan

Chapters

  • 00:05Title
  • 01:10Colon Tumor Isolation and Cell Dissociation
  • 02:52Culture of Intestinal Tumor
  • 03:41Maintenance of Established Organoids
  • 04:36Storage and Recovery of Established Organoids
  • 05:23RNA Extraction, Protein Extraction and Immunohistochemistry
  • 06:12Results: Formation of Organoids
  • 07:13Conclusion

Summary

Automatic Translation

Automatic Translation

En simpel metode til at etablere den primære murine colontumor organoid beskrives. Denne metode udnytter den funktion, der colontumorceller overleve og vokse ind organoids i medier indeholder begrænsede vækstfaktorer, mens normal colon epitel ikke.

Tags

In Vitro Organoid CulturePrimary Mouse Colon TumorsColon Cancer Cell LinesPhysiologic IntegrityBasal-apical PolarityAnoikisTumor OrganoidsGenetically Modified MiceGenetic ManipulationSignaling PathwaysColon Tumorigenesis

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