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In vitro Organoid Culture of Primary Mouse Colon Tumors
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JoVE Journal Medicine
In vitro Organoid Culture of Primary Mouse Colon Tumors

In vitro Organoid Culture of Primary Mouse Colon Tumors

DOI:
10.3791/50210-v

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07:33 min

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May 17, 2013

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,
1Department of Molecular & Integrative Physiology,University of Michigan , 2Department of Internal Medicine, Division of Gastroenterology,University of Michigan

Chapters

  • 00:05Title
  • 01:10Colon Tumor Isolation and Cell Dissociation
  • 02:52Culture of Intestinal Tumor
  • 03:41Maintenance of Established Organoids
  • 04:36Storage and Recovery of Established Organoids
  • 05:23RNA Extraction, Protein Extraction and Immunohistochemistry
  • 06:12Results: Formation of Organoids
  • 07:13Conclusion

Summary

Automatic Translation

Automatic Translation

A simple method to establish primary murine colon tumor organoid is described. This method utilizes the feature that colon tumor cells survive and grow into organoids in media containing limited growth factors, whereas normal colon epithelial do not.

Tags

In Vitro Organoid CulturePrimary Mouse Colon TumorsColon Cancer Cell LinesPhysiologic IntegrityBasal-apical PolarityAnoikisTumor OrganoidsGenetically Modified MiceGenetic ManipulationSignaling PathwaysColon Tumorigenesis

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