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JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Generation of a Three-dimensional Full Thickness Skin Equivalent and Automated Wounding
DOI:

08:35 min

February 26, 2015

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Chapters

  • 00:05Title
  • 01:55Production of Dermal Component
  • 03:36Addition of Human Epidermal Keratinocytes and Culture of Full Thickness Skin Equivalents
  • 05:14Injury with Automated Wounding Device
  • 06:19Results: (Immuno-)Histological Analysis and Wounding of Full Thickness Skin Equivalents
  • 08:10Conclusion

Summary

Automatic Translation

The goal of this protocol is to build up a three-dimensional full thickness skin equivalent, which resembles natural skin. With a specifically constructed automated wounding device, precise and reproducible wounds can be generated under maintenance of sterility.

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