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JoVE Journal Bioengineering
Engineering Three-dimensional Epithelial Tissues Embedded within Extracellular Matrix
 

Engineering Three-dimensional Epithelial Tissues Embedded within Extracellular Matrix

Article DOI: 10.3791/54283-v • 08:49 min • July 10th, 2016
July 10th, 2016 •
1, 1,2
1Chemical and Biological Engineering, Princeton University, 2Molecular Biology, Princeton University

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Summary
Automatic Translation

This manuscript describes a soft lithography-based technique to engineer uniform arrays of three-dimensional (3D) epithelial tissues of defined geometry surrounded by extracellular matrix. This method is amenable to a wide variety of cell types and experimental contexts and allows for high-throughput screening of identical replicates.

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Keywords: 3D Epithelial Tissues Extracellular Matrix Tissue Engineering Branching Morphogenesis Collective Cell Behavior Photolithography PDMS Mold Stamp Tissue Culture
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