Induced pluripotent stem cells (iPSCs) are somatic cells that have been genetically reprogrammed to form undifferentiated stem cells. Like embryonic stem cells, iPSCs can be grown in culture conditions that promote differentiation into different cell types. Thus, iPSCs may provide a potentially unlimited source of any human cell type, which is a major breakthrough in the field of regenerative medicine. However, more research into the derivation and differentiation of iPSCs is still needed to actually use these cells in clinical practice.
This video first introduces the fundamental principles behind cellular reprogramming, and then demonstrates a protocol for the generation of iPSCs from differentiated mouse embryonic fibroblasts. Finally, it will discuss several experiments in which scientists are improving or applying iPSC generation techniques.
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Induced pluripotent stem cells, like human embryonic stem cells, can differentiate into almost any cell in the body, and therefore hold great promise in the field of regenerative medicine. Human embryonic stem cells, or hESCs, are obtained from pre-implantation embryos, whereas fully differentiated somatic cells are used to generate induced pluripotent stem cells, which are also referred to as iPSCs. In this video, you are going to learn about the basic principles behind generating iPSCs, a step-by-step p…