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Vurdering Retinal mikrogliaceller fagocytotisk funktion<em> In vivo</em> Brug en flowcytometri-baseret assay
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Assessing Retinal Microglial Phagocytic Function In Vivo Using a Flow Cytometry-based Assay
DOI:

07:19 min

October 18, 2016

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Chapters

  • 00:05Title
  • 00:47Intravitreal Bead Injection
  • 02:48Retinal Tissue Harvest
  • 04:19Single Cell Suspension Staining for Flow Cytometric Analysis
  • 05:49Results: Representative Microglia Phagocytic Function after LPS Challenge in Pup and Adult Mice
  • 06:30Conclusion

Summary

Automatic Translation

Mikroglial fagocytose er kritisk for opretholdelse af vævshomeostase og utilstrækkelig fagocyterende funktion er blevet impliceret i patologien. Men vurderer mikroglia funktion in vivo er teknisk udfordrende. Vi har udviklet en enkel men robust teknik til præcis overvågning og kvantificering af fagocytiske potentiale mikroglia i et fysiologisk miljø.

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