Rivista
/
/
CRISPR/Cas9 蛋白注射到沟鲶、鮰松毛虫、基因编辑的胚胎
JoVE Journal
Genetica
Author Produced
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Genetica
Microinjection of CRISPR/Cas9 Protein into Channel Catfish, Ictalurus punctatus, Embryos for Gene Editing
DOI:

16:08 min

January 20, 2018

, , , , , , ,

Capitoli

  • 00:00Titolo
  • 01:14Brood Stock Selection and Spawning
  • 04:16Sperm Preparation
  • 05:33Egg Collection and Fertilization
  • 08:07Needle Pulling and Loading
  • 10:20Microinjection of Catfish Embryos
  • 12:34Risultati
  • 15:24Conclusion

Summary

Traduzione automatica

本文介绍了一种简单高效的 CRISPR/Cas9 系统在沟鲶胚胎基因编辑中的微注射协议。在这个协议中, 引导 rna 和 Cas9 蛋白被微量到一细胞胚胎的卵黄中。通过敲出两条沟鲶免疫相关基因来验证这个协议。

Video correlati

Read Article