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Laser fange Microdissection av svært ren trabekulært Meshwork fra musen øyne for Gene Expression analyse
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JoVE Journal Biology
Laser Capture Microdissection of Highly Pure Trabecular Meshwork from Mouse Eyes for Gene Expression Analysis

Laser fange Microdissection av svært ren trabekulært Meshwork fra musen øyne for Gene Expression analyse

DOI:
10.3791/57576-v

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13:47 min

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June 03, 2018

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, , , , , , ,
1Immunity, Inflammation, and Disease Laboratory, Division of Intramural Research,National Institute of Environmental Health Sciences, NIH, 2Cellular and Molecular Pathology Branch, Division of the National Toxicology Program,National Institute of Environmental Health Sciences, NIH, 3Integrated Laboratory Systems Inc., 4Experimental Pathology Laboratories Inc.

Chapters

  • 00:05Title
  • 01:00Frozen Section Preparation for Laser Microdissection
  • 04:25Polyethylene Terephthalate (PET) Membrane Slides Processing and Staining Protocol
  • 06:14Laser Microdissection with UV Laser
  • 11:01Results: Evaluation of RNA Quality and Downstream RNA Analysis
  • 12:25Conclusion

Summary

Automatic Translation

Automatic Translation

Her beskriver vi en reproduserbar laser fange microdissection (LCM) er en protokoll for å isolere trabekulært meshwork (TM) for nedstrøms RNA analyse. Muligheten til å analysere endringer i byggkorn under TM vil hjelpe forstå underliggende molekylære mekanismer av TM-relaterte okulær sykdommer.

Tags

Laser MicrodissectionTrabecular MeshworkGene Expression AnalysisOcular DiseasesRNA IsolationCryostat SectioningH&E StainingLaser Capture Microscopy

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