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Laser Capture Microdissection of Highly Pure Trabecular Meshwork from Mouse Eyes for Gene Expression Analysis
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Laser Capture Microdissection of Highly Pure Trabecular Meshwork from Mouse Eyes for Gene Expression Analysis

Laser Capture Microdissection of Highly Pure Trabecular Meshwork from Mouse Eyes for Gene Expression Analysis

DOI:
10.3791/57576-v

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13:47 min

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June 03, 2018

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, , , , , , ,
1Immunity, Inflammation, and Disease Laboratory, Division of Intramural Research,National Institute of Environmental Health Sciences, NIH, 2Cellular and Molecular Pathology Branch, Division of the National Toxicology Program,National Institute of Environmental Health Sciences, NIH, 3Integrated Laboratory Systems Inc., 4Experimental Pathology Laboratories Inc.

Chapters

  • 00:05Title
  • 01:00Frozen Section Preparation for Laser Microdissection
  • 04:25Polyethylene Terephthalate (PET) Membrane Slides Processing and Staining Protocol
  • 06:14Laser Microdissection with UV Laser
  • 11:01Results: Evaluation of RNA Quality and Downstream RNA Analysis
  • 12:25Conclusion

Summary

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Here, we describe a protocol for a reproducible laser capture microdissection (LCM) for isolating trabecular meshwork (TM) for downstream RNA analysis. The ability to analyze changes in gene expression in the TM will help in understanding the underlying molecular mechanisms of TM-related ocular diseases.

Tags

Laser MicrodissectionTrabecular MeshworkGene Expression AnalysisOcular DiseasesRNA IsolationCryostat SectioningH&E StainingLaser Capture Microscopy

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