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Isolering, ekspansion og differentiering af mesenkymale stamceller fra den infrapatellære fedtpude i gedekvæleleddet
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Isolation, Expansion, and Differentiation of Mesenchymal Stem Cells from the Infrapatellar Fat Pad of the Goat Stifle Joint
DOI:

12:06 min

August 02, 2022

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Chapters

  • 00:05Introduction
  • 01:08Isolation of Infrapatellar Fat Pad Mesenchymal Stem Cells (IFP-MSCs) from the Femorotibial Joint of Goat Knee
  • 05:16Maintenance and Expansion of Isolated Cells
  • 07:13Differentiation Potential of IFP-MSCs
  • 08:49Results: Expansion, Clonogenic Ability, and Differentiation Potential of IFP-MSCs
  • 11:07Conclusion

Summary

Automatic Translation

Infrapatellar fedtpude mesenkymale stamceller (IFP-MSC'er) kan let isoleres fra knæleddets infrapatellære fedtpude. De formerer sig godt in vitro, danner CFU-F-kolonier og differentierer sig til adipogene, kondrogene og osteogene slægter. Heri gives metoden til isolering, udvidelse og differentiering af IFP-MSC'er fra gedekvælningsled.

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