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JoVE Journal
Bioengineering

This content is Open Access.

Optimizing Attachment of Human Mesenchymal Stem Cells on Poly(ε-caprolactone) Electrospun Yarns
 

Optimizing Attachment of Human Mesenchymal Stem Cells on Poly(ε-caprolactone) Electrospun Yarns

Article DOI: 10.3791/52135-v 10:50 min April 10th, 2015
April 10th, 2015

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Summary

This article describes a range of set-ups for seeding human mesenchymal stem cells onto materials, in this case electrospun yarns, that do not cover the base of standard culture well plates in order to maximize and quantify the number of cells that initially attach compared to the known seeding density.

Tags

Keywords: Mesenchymal Stem Cells Poly(ε-caprolactone) Electrospun Yarns Cell Attachment Cell Culture Bioreactor Cell Culture Insert PTFE Trough Shaking Motion
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