Rivista
/
Biologia
/
Channelrhodopsin2介导的突触电位刺激果蝇神经肌肉接头
JoVE Journal
Biologia
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Biologia
Channelrhodopsin2 Mediated Stimulation of Synaptic Potentials at Drosophila Neuromuscular Junctions
Please note that all translations are automatically generated. Click here for the English version.

Channelrhodopsin2介导的突触电位刺激果蝇神经肌肉接头

DOI:
10.3791/1133-v

•

09:12 min

•

March 16, 2009

•

, ,
1Department of Biology,Brandeis

Capitoli

  • 00:00Titolo
  • 00:45Introduction
  • 01:14Animal Care and Genetic Crosses
  • 02:48Electrophysiology Rig Setup
  • 04:03Larval Dissection
  • 06:37Blue Light Stimulation and Muscle Recording
  • 07:54Representative Results: Light Induced E.J.P.s
  • 08:28Conclusion

Summary

Traduzione automatica

Traduzione automatica

此过程使用蓝色光激活藻通道和细胞特异性基因表达的工具，以唤起光脉冲在神经肌肉接头（NMJ）突触潜力<em>果蝇</em>幼虫。这种技术是一种廉价和易于使用替代吸电极刺激突触生理学研究，在研究和教学实验室。

Tags

Channelrhodopsin2StimulationSynaptic PotentialsDrosophila Neuromuscular JunctionsLarval PreparationExcitatory Junctional PotentialsSuction ElectrodesRemote StimulationMotor NeuronsGAL4-UAS SystemElectrophysiology RigBlue LightNeurophysiology Teaching Labs

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

check_url/it/1133?article_type=v

载入果蝇与神经末梢钙指标
check_url/it/1133?article_type=v

果蝇在操作性学习扭力计
check_url/it/1133?article_type=v

一个磁性的系绳系统，以调查在果蝇的视觉和嗅觉介导的飞行控制
check_url/it/1133?article_type=v

电生理方法记录从果蝇幼虫NMJ突触潜力
check_url/it/1133?article_type=v

在果蝇的飞行过程中可视介导的气味追踪
check_url/it/1133?article_type=v

可视化的Live果蝇胶质细胞神经​​肌肉交界处，与荧光染料
check_url/it/1133?article_type=v

线虫神经肌肉交界处的解剖和录制
check_url/it/1133?article_type=v

在完整的果蝇幼虫体内的亚细胞分辨率成像
check_url/it/1133?article_type=v

定量比较顺调节元件（CRE）的活动，在转基因果蝇</em
check_url/it/1133?article_type=v

重组工程同源重组构建<em>果蝇</em

Read Article