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Standardized Preparation of Single-Cell Suspensions from Mouse Lung Tissue using the gentleMACS Dissociator
 

Standardized Preparation of Single-Cell Suspensions from Mouse Lung Tissue using the gentleMACS Dissociator

Article DOI: 10.3791/1266-v • 07:04 min • July 2nd, 2009
July 2nd, 2009 •
1, 1, 1, 1, 1
1Miltenyi Biotec,GmbH

Capitoli

Riepilogo
Automatic Translation

Dissociating cells from specific tissue types requires specific parameters for tissue agitation to obtain a high volume of viable, culturable cells. The Miltenyi gentleMACS dissociator optimizes this task with a simple, practical protocol. In this publication the use of this apparatus on lung tissue is explained.

Tags

Standardized Preparation Single-cell Suspensions Mouse Lung Tissue GentleMACS Dissociator Experiments Cellular Research Dissociating Whole Organs Viable Cells Reproducible Manner Instrument Pre-programmed Settings Optimized Efficient Dissociation Tissue Types Mouse Lungs Publication
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