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制备海兔感觉运动神经元细胞培养

JoVE Journal
Biologia

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JoVE Journal Biologia

Preparation of Aplysia Sensory-motor Neuronal Cell Cultures

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制备海兔感觉运动神经元细胞培养

DOI:

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17:27 min

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June 08, 2009

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Yali Zhao, Dan O. Wang, Kelsey C. Martin^2,3

¹Dept. of Psychiatry and Biobehavioral Sciences,University of California, Los Angeles, ²Dept. of Biological Chemistry,University of California, Los Angeles, ³Semel Institute for Neuroscience and Human Behavior,University of California, Los Angeles

Capitoli

00:05Titolo
00:37Introduction
01:22Preparation
03:42Preparation of Hemolymph
05:33Dissecting the Ganglia from Aplysia
10:05Removing and Culturing Neurons from the Pleural Ganglia
12:25Isolating Motor Neurons and Preparing Co-cultures with Sensory Neurons
16:53Conclusion

Summary

Traduzione automatica

小学文化<em>海兔</em>感觉运动神经元突触的形成和突触可塑性研究提供一个模型的制备<em>在体外</em>。这个视频演示的感觉和运动神经元的鉴定和显微切割<em>海兔</em>神经节以及建立和保持文化的感觉运动神经元的方法。

Tags

Aplysia Sensory-motor Neuronal Cell Cultures Marine Mollusk Aplysia Californica Neurons Ganglia Circuitry Gill-withdrawal Reflex Tactile Stimulation Synaptic Plasticity Serotonin Short-term Facilitation Intermediate-term Facilitation Long-term Facilitation Peptide Transmitter FMRFamide Synaptic Weakening

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