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Fluorescent Labeling of Drosophila Heart Structures
 

Fluorescent Labeling of Drosophila Heart Structures

Article DOI: 10.3791/1423-v • 11:12 min • October 13th, 2009
October 13th, 2009 •
1,2, 2, 2, 2, 2, 1,2
1Biology Department, San Diego State University, 2Development and Aging Program, NASCR Center, The Sanford Burnham Institute for Medical Research

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Here we describe a basic protocol for fluorescent labeling of different elements of heart tubes from larva and adult Drosophila melanogaster. These specimens are well-suited for imaging via fluorescent or confocal microscopy. This technique permits detailed structural analysis of the features of the hearts from a powerful model organism.

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Fluorescent Labeling Drosophila Heart Structures Drosophila Melanogaster Dorsal Vessel Cardiomyocytes Pericardial Cells Alary Muscles Ventral Longitudinal Muscle Cells Myofibrils Cytoskeletal/submembranous Protein Complexes Extracellular Matrix Fixation Fluorescent Labeling Protocol Sarcomeric F-actin α-actinin Myosin-GFP Expressing Adult Flies Pericardin Type IV Extracellular Matrix Collagen Mounting Strategy Semi-intact Adult Hearts Imaging Confocal Microscopy
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