È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
Capitoli
- 00:00Titolo
- 00:37Introduction
- 01:18Isolation of Adult Cardiac Myocytes from Rat Heart by Langendorrf Preparation
- 08:02Primary Cell Culture and Adenoviral Transduction
- 09:45Confocal Imaging of 3D Subcellular Structures
- 11:09Imaging of Adult Rat Cardiac Myocytes Representative Results
- 11:43Conclusion
Adult cardiac myocytes are primary cells that can be isolated from animal hearts and cultured for several days. Within this culture period adenoviral gene transfer can be used to express genetically encoded biosensors (GEBs) or fluorescent fusion proteins. Both approaches allow cellular investigations by means of confocal microscopy.
Tags
IsolationGenetic ManipulationAdult Cardiac MyocytesConfocal ImagingEmbryonic CellsNeonatal Muscle CellsWorking HeartCardiac Cellular PhysiologyPathophysiologyPharmaceutical InvestigationsTransgenic Animal ModelsLong Term CultureAdenoviral Gene TransferGenetically Encoded BiosensorsFluorescent Fusion ProteinsProtein OverexpressionProtein DownregulationRNAiSubcellular Structure StainingGolgi VisualizationConfocal ImagingZ-stacks ReconstructionCellular Cardiology
