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Capitoli
- 00:00Titolo
- 00:57Introduction
- 01:29Transfection of Plasmids for Production of Renilla-antigen Fusion Proteins
- 03:28Harvesting Renilla-antigen Fusions
- 05:12Preparing a Sera Master Plate
- 06:30LIPS Assay
- 09:01Measuring Luminescence and Data Analysis
- 10:49LIPS Assay as a Method for Rapid and High Thoughput Sera Antigen Analysis
- 11:25Conclusion
Os aspectos técnicos do desempenho LIPS (Sistemas de imunoprecipitação luciferase) são descritos. A abordagem global envolve expressar genes quiméricos codificando antígenos fundido a Renilla luciferase (Ruc) em células de mamíferos. Crude Ruc-antígeno extratos são então preparadas e, sem a purificação, empregada em ensaios de imunoprecipitação para quantificar anticorpos.
Tags
Antibody ProfilingLuciferase Immunoprecipitation Systems (LIPS)Comprehensively UnderstandingQuantifying Antibody ProfilesAutoantigensInfectious AgentsDisease MechanismsMarkersSubstratify DiseaseClinical FeaturesPathogenesisHighly Quantitative MethodPatient Sera Antibody ResponsesPathogen AntigensInfectionELISAsHumoral Serological Response ProfilesUniversal FormatDynamic Antibody Titer RangesProteomeHIVInformativeSingle Antigen ELISAPurified AntigensAssay Optimization Steps
