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Cryotomography électrons des cellules bactériennes
JoVE Journal
Biologia
This content is Free Access.
JoVE Journal Biologia
Electron Cryotomography of Bacterial Cells
DOI:

14:23 min

May 06, 2010

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Capitoli

  • 00:00Titolo
  • 00:39Introduction
  • 02:11Plunge Freezing Thin Bacterial Cells
  • 03:41High Pressure Freezing and Cryo Sectioning of Thicker Cells
  • 06:43Examination of the Cells Using the Cryo Light Microscope
  • 07:49Collecting Tilt Series Using the Cryo TEM
  • 09:08Tomogram Reconstruction and Database
  • 10:24Tomogram Segmentation
  • 10:51Movies and Animation
  • 11:22Representative Movies
  • 12:59Conclusion

Summary

Traduzione automatica

Nous illustrons ici comment utiliser électrons cryotomography (ECT) pour étudier l'ultrastructure des cellules bactériennes en quasi-natif états, à «macromoléculaires" (~ 4 nm) de résolution.

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