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Quantification of γH2AX Foci in Response to Ionising Radiation
 
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Quantification of γH2AX Foci in Response to Ionising Radiation

Article DOI: 10.3791/1957-v • 06:54 min • April 6th, 2010
April 6th, 2010 •
1,2, 1,2,3, 1,3, 1, 2,3, 1,2
1Epigenomic Medicine, Baker IDI Heart and Diabetes Institute, The Alfred Medical Research and Education Precinct, 2Department of Pathology, The University of Melbourne, 3Epigenetics in Human Health and Disease, Baker IDI Heart and Diabetes Institute, The Alfred Medical Research and Education Precinct

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Quantification of DNA double-strand streaks using γH2AX formation as a molecular marker has become an invaluable tool in radiation biology. Here we demonstrate the use of an immunofluorescence assay for quantification of γH2AX foci after exposure of cells to radiation.

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γH2AX Foci Quantification Ionising Radiation DNA Double-strand Breaks H2A Histone Variant Phosphorylation Serine-139 Residue C-terminal SQEY Motif Phosphatidyl-inosito 3-kinase (PIKK) Family Ataxia Telangiectasia Mutated (ATM) DNA-protein Kinase Catalytic Subunit ATM And RAD3-related (ATR) Chromatin Modifications DNA Damage Signalling And Repair Immunofluorescence Microscopy Repair Of DSBs
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