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Preparation of Drosophila S2 cells for Light Microscopy
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JoVE Journal Biologia
Preparation of Drosophila S2 cells for Light Microscopy

Preparation of Drosophila S2 cells for Light Microscopy

DOI:
10.3791/1982-v

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09:39 min

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June 03, 2010

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, , ,
1Department of Cell Biology and Anatomy,University of Arizona (UOA)

Capitoli

  • 00:00Titolo
  • 00:51Introduction
  • 01:28Preparation of Glass-Bottom Dishes
  • 04:16Preparation of S2 Cells for Live Cell Microscopy
  • 05:12Fixation and Immunostaining of S2 Cells in the Glass-bottom Dish
  • 07:50Results of Microscopy of S2 Cells
  • 09:21Conclusion

Summary

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Drosophila Schneider (S2) cells are an increasingly popular system for the discovery and functional analysis of genes. Our goal is to describe some of the microscopic techniques that make S2 cells such an increasingly important experimental system.

Tags

Drosophila S2 CellsLight MicroscopyExperimental SystemTechniquesLiteratureGenome Sequence DatabaseCellular ProcessesGenesMolecular ComponentsRNAiFluorescence MicroscopyLive CellsFixed CellsMediaSerum-free MediaTemperatureContainersAtmosphereSealed FlasksPhenotypes

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