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Isolation and Magnetic Separation of Murine CD34⁺ Stem Cells from the Vascular Wall

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Murine Aortal and Mesenteric Arteries Isolation and Culture to Separate CD34⁺ Stem Cells for Cardiovascular Studies

JoVE Journal
Medicina

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JoVE Journal Medicina

Murine Aortal and Mesenteric Arteries Isolation and Culture to Separate CD34⁺ Stem Cells for Cardiovascular Studies

Murine Aortal and Mesenteric Arteries Isolation and Culture to Separate CD34⁺ Stem Cells for Cardiovascular Studies

Isolation and Magnetic Separation of Murine CD34⁺ Stem Cells from the Vascular Wall

DOI:

10.3791/201053-v

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01:36 min

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December 22, 2023

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Chuting Han, Cheng Xie, Qingya Dang, Xiaolin Zhang, Chunshu Li, Yan Yang, Jun Cheng, Pengyun Li

¹Key Laboratory of Medical Electrophysiology of Ministry of Education and Medical Electrophysiological Key Laboratory of Sichuan Province, Institute of Cardiovascular Research,Southwest Medical University

Tags

Immunomagnetic Isolation Vascular Wall-resident CD34+ Stem Cells Purification Flow Cytometry Functional Identification Immunofluorescence Staining Ca2+ Imaging Murine Aorta And Mesenteric Artery Adventitia Cell Culture Multipotency Differentiation ATP Caffeine Thapsigargin

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