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一步代谢：碳水化合物，在一个单一的过程量化的有机和氨基酸
JoVE Journal
Biologia
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JoVE Journal Biologia
One-step Metabolomics: Carbohydrates, Organic and Amino Acids Quantified in a Single Procedure
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一步代谢：碳水化合物，在一个单一的过程量化的有机和氨基酸

DOI:
10.3791/2014-v

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09:28 min

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June 25, 2010

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1Edward A. Doisy Department of Biochemistry and Molecular Biology,Saint Louis University School of Medicine

Capitoli

  • 00:04Titolo
  • 00:55Introduction
  • 01:58Removal of Urea from Urine Sample
  • 04:05Dehydration & Derivatization then GC/MS
  • 07:14Representative Results
  • 08:12Conclusion

Summary

Traduzione automatica

Traduzione automatica

脲中介代谢物的GC / MS分析的样品制备方法是由它的发明者。该方法允许单步跟进串联质谱新生儿筛查先天的错误量化碳水化合物，有机和氨基酸都在一个单一的过程。

Tags

One-step MetabolomicsCarbohydratesOrganic AcidsAmino AcidsTandem Mass SpectrometryAcylcarnitinesOrganic AcidemiasAmino Acid QuantificationEnzymatic TestingCarbohydrate DisordersUrease MethodSample PreparationGas ChromatographyDehydrationDerivatizationTrimethylsilylationCatalystTriethylammonium TrifluoroacetateAutomated InjectionChromatographyQuantification Of MetabolitesSemi-quantificationMass SpectraTMS Derivatized Biological Compounds

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