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视网膜色素上皮的生理与病理生理学研究的实验模型
JoVE Journal
Neuroscienze
This content is Free Access.
JoVE Journal Neuroscienze
Experimental Models for Study of Retinal Pigment Epithelial Physiology and Pathophysiology
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视网膜色素上皮的生理与病理生理学研究的实验模型

DOI:
10.3791/2032-v

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08:28 min

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November 06, 2010

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,
1National Eye Institute,National Institutes of Health

Capitoli

  • 00:00Titolo
  • 01:08Introduction
  • 02:13Background on Retinal Pigment Epithelium (RPE)
  • 03:26Eye Dissection
  • 05:57Isolation and Dissociation of Human Fetal Retinal Pigment Epithelium (hfRPE)
  • 08:07Conclusion

Summary

Traduzione automatica

Traduzione automatica

我们提供了一个汇合单层培养的人胚胎视网膜色素上皮细胞（hfRPE）细胞重现的方法，表现出形态，生理，极性，和成人自然组织的蛋白质和基因表达模式。这项工作已经延伸到一些眼部疾病的动物模型。

Tags

Retinal Pigment EpitheliumPhysiologyPathophysiologyCell Culture ProcedurePrimary Human Fetal Retinal Pigment EpitheliumHfRPE Cell CulturesPigmentationElectron MicroscopyApical Membrane MicrovilliTight Junction ProteinsEpithelial PolarityFunctionMammalian ModelsTherapeutic InterventionsAnimal ModelsEye DiseaseAbnormal Fluid AccumulationSubretinal SpacePhotoreceptor Outer SegmentsRetinal AttachmentsRPE/retina Interactions

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