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Studying the Effects of Matrix Stiffness on Cellular Function using Acrylamide-based Hydrogels

JoVE Journal
Biologia

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JoVE Journal Biologia

Studying the Effects of Matrix Stiffness on Cellular Function using Acrylamide-based Hydrogels

Studying the Effects of Matrix Stiffness on Cellular Function using Acrylamide-based Hydrogels

DOI:

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10:19 min

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August 10, 2010

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Alexandra Cretu, Paola Castagnino, Richard Assoian

¹Department of Pharmacology,University of Pennsylvania

Capitoli

00:04Titolo
00:49Introduction
01:10Coverslip Preparation
03:50Preparation of Hydrogels
06:15Modeling
08:35Representative Results
09:50Conclustion

Summary

Traduzione automatica

The effect of substrata stiffness on cellular function can be modeled in vitro using polyacrylamide hydrogels of varying compliances.

Tags

Matrix Stiffness Cellular Function Acrylamide-based Hydrogels Fibrosis Cancer Cardiovascular Disease Cell Biology ECM-coated Hydrogels Polyacrylamide NaOH 3-APTMS Glutaraldehyde Acrylamide (AC)Bis-acrylamide (Bis-AC)Ammonium Persulfate N-hydroxysuccinimide (NHS)Rheology Atomic Force Microscopy (AFM)

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