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Generation of Transgenic C. elegans by Biolistic Transformation
 
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Generation of Transgenic C. elegans by Biolistic Transformation

Article DOI: 10.3791/2090-v • 10:15 min • August 23rd, 2010
August 23rd, 2010 •
1, 1, 1
1Department of Medicine, University of Pittsburgh

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Transgenic worms are commonly used in C. elegans research. Described is a simple, yet effective, protocol to introduce transgenes into worms using biolistic bombardment with DNA-coated gold particles. The effort involved and results of bombardment compare favorably with microinjection for the generation of transgenic animals.

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Transgenic C. Elegans Biolistic Transformation Nematode Model Worm Research Gene Localization Function Study Transgenic Animals Microinjection Germline Biolistic Bombardment Gold Particles Bio-Rad PDS-1000 System Multiple Transgenic Lines Extrachromosomal Arrays Integration
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