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Measuring RAN Peptide Toxicity in C. elegans
JoVE Journal
Biologia
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JoVE Journal Biologia
Measuring RAN Peptide Toxicity in C. elegans
DOI:

10:49 min

April 30, 2020

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Capitoli

  • 00:04Introduction
  • 01:05Measurement of the Developmental Toxicity of RAN Peptides following RNAi-based Gene Knockdown
  • 05:38Post-developmental RAN Peptide Paralysis Assay
  • 08:02Results: Toxicity of RAN Peptides
  • 10:04Conclusion

Summary

Traduzione automatica

Repeat-associated non-ATG-dependent translational products are emerging pathogenic features of several repeat expansion-based diseases. The goal of the protocol described is to evaluate toxicity caused by these peptides using behavioral and cellular assays in the model system C. elegans.

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