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单细胞转染鸡胚

JoVE Journal
Biologia

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JoVE Journal Biologia

Single Cell Transfection in Chick Embryos

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单细胞转染鸡胚

DOI:

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08:46 min

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September 25, 2010

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Raz Ben-Yair, Chaya Kalcheim

¹Department of Medical Neurobiology,Hadassah Medical School – Hebrew University

Capitoli

00:00Titolo
00:57Introduction
01:21Micropipette, DNA, and Embryo Preparation
04:15Loading Micropipettes and Microinjection
06:49Representative Injection Results
08:18Conclusion

Summary

Traduzione automatica

使用精尖微量注入鸡体节或神经管的子域的质粒DNA。质粒的浓度调整，以产生单一的转染细胞。然后，我们允许的细胞发育成无性系种群。

Tags

Single Cell Transfection Chick Embryos Biologia dello sviluppo Lineage Diversification Molecular Mechanisms Transfection Methods Single Progenitors In-ovo Transfection Lineage Tracing Genetic Manipulation Somite Neural Tube Epithelium Sporadic Transfection Clonal Populations Reporters Genetic Manipulation Tools Electroporation Method In-vivo Models

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