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Injection of dsRNA into Female A. aegypti Mosquitos
 

Injection of dsRNA into Female A. aegypti Mosquitos

Article DOI: 10.3791/215-v • 04:25 min • July 4th, 2007
July 4th, 2007 •
1, 1, 2
1Department of Molecular Biology and Biochemistry, University of California, Irvine (UCI), 2Department of Molecular Biology and Biochemistry, Department of Microbiology and Molecular Genetics, University of California, Irvine (UCI)

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Reverse genetic approaches have proven extremely useful for determining which genes underly resistance to vector pathogens in mosquitoes. This video protocol illustrates a method used by the James lab to inject dsRNA into female A. aegypti mosquitoes, which harbor the dengue virus. The technique for calibrating injection needles, manipulating the injection setup, and injecting dsRNA into the thorax is illustrated.

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DsRNA Injection Reverse Genetic Approach A. Aegypti Mosquitoes Vector Pathogens James Lab Dengue Virus Calibrating Injection Needles Manipulating Injection Setup Injecting DsRNA Thorax
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