Rivista
/
Biologia
/
Injection of dsRNA into Female A. aegypti Mosquitos
JoVE Journal
Biologia
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Biologia
Injection of dsRNA into Female A. aegypti Mosquitos

Injection of dsRNA into Female A. aegypti Mosquitos

DOI:
10.3791/215-v

•

04:24 min

•

July 04, 2007

•

, ,
1Department of Molecular Biology and Biochemistry,University of California, Irvine (UCI), 2Department of Molecular Biology and Biochemistry, Department of Microbiology and Molecular Genetics,University of California, Irvine (UCI)

Capitoli

  • 00:07Introduction
  • 00:37Preparation of Needles for Injection of dsRNA
  • 01:35The Injection Setup
  • 02:47Injection of dsRNA
  • 03:55Conclusion
  • 04:17End Credits

Summary

Traduzione automatica

Traduzione automatica

Reverse genetic approaches have proven extremely useful for determining which genes underly resistance to vector pathogens in mosquitoes. This video protocol illustrates a method used by the James lab to inject dsRNA into female A. aegypti mosquitoes, which harbor the dengue virus. The technique for calibrating injection needles, manipulating the injection setup, and injecting dsRNA into the thorax is illustrated.

Tags

DsRNA InjectionReverse Genetic ApproachA. Aegypti MosquitoesVector PathogensJames LabDengue VirusCalibrating Injection NeedlesManipulating Injection SetupInjecting DsRNAThorax

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

check_url/it/215?article_type=v

Microinjection of A. aegypti Embryos to Obtain Transgenic Mosquitoes
check_url/it/215?article_type=v

Protocol for Dengue Infections in Mosquitoes (A. aegypti) and Infection Phenotype Determination
check_url/it/215?article_type=v

Dissection of Midgut and Salivary Glands from Ae. aegypti Mosquitoes
check_url/it/215?article_type=v

Intraperitoneal Injection into Adult Zebrafish
check_url/it/215?article_type=v

Direct Delivery of MIF Morpholinos Into the Zebrafish Otocyst by Injection and Electroporation Affects Inner Ear Development
check_url/it/215?article_type=v

RNAi Interference by dsRNA Injection into Drosophila Embryos
check_url/it/215?article_type=v

RNAi-mediated Gene Knockdown and In Vivo Diuresis Assay in Adult Female Aedes aegypti Mosquitoes
check_url/it/215?article_type=v

Measuring Peptide Translocation into Large Unilamellar Vesicles
check_url/it/215?article_type=v

Physiological Recordings and RNA Sequencing of the Gustatory Appendages of the Yellow-fever Mosquito Aedes aegypti
check_url/it/215?article_type=v

RNAi Trigger Delivery into Anopheles gambiae Pupae

Read Article