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女答：埃及伊蚊蚊的dsRNA注入
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Biologia
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JoVE Journal Biologia
Injection of dsRNA into Female A. aegypti Mosquitos
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女答：埃及伊蚊蚊的dsRNA注入

DOI:
10.3791/215-v

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04:24 min

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July 04, 2007

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, ,
1Department of Molecular Biology and Biochemistry,University of California, Irvine (UCI), 2Department of Molecular Biology and Biochemistry, Department of Microbiology and Molecular Genetics,University of California, Irvine (UCI)

Capitoli

  • 00:07Introduction
  • 00:37Preparation of Needles for Injection of dsRNA
  • 01:35The Injection Setup
  • 02:47Injection of dsRNA
  • 03:55Conclusion
  • 04:17End Credits

Summary

Traduzione automatica

Traduzione automatica

反向遗传方法已被证明非常有用，确定哪些基因底层电阻载体病原体在蚊子。这个视频协议，说明了由詹姆斯实验室用于注入雌性埃及伊蚊的蚊子，海港登革热病毒的双链RNA的方法。校准注射针头，注射设置进行操作，并注入胸廓的dsRNA的技术说明。

Tags

DsRNA InjectionReverse Genetic ApproachA. Aegypti MosquitoesVector PathogensJames LabDengue VirusCalibrating Injection NeedlesManipulating Injection SetupInjecting DsRNAThorax

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