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小学组织两个胸苷类似物的免疫荧光检测（CldU IDU）
JoVE Journal
Biologia
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JoVE Journal Biologia
Immunofluorescent Detection of Two Thymidine Analogues (CldU and IdU) in Primary Tissue
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小学组织两个胸苷类似物的免疫荧光检测（CldU IDU）

DOI:
10.3791/2166-v

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10:55 min

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December 07, 2010

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, , , , , , , , , , ,
1Division of Endocrinology and Diabetes, Children’s Hospital of Philadelphia, Institute of Diabetes Obesity and Metabolism, Institute for Regenerative Medicine, Department of Pediatrics,University of Pennsylvania-School of Medicine

Capitoli

  • 00:01Titolo
  • 02:53Primary and Secondary Antibodies
  • 09:35Imaging and Analysis

Summary

Traduzione automatica

Traduzione automatica

我们派生的策略，以检测到成年小鼠组织中的胸苷类似物的顺序法团（CldU IDU），以量化的细胞分裂的两个连续两轮。这一战略是有用的检测长寿命的组织细胞的营业额，致癌基因改造，或过境扩增细胞。

Tags

Immunofluorescent DetectionThymidine AnaloguesCldUIdUCell Division MeasurementExperimental BiologyMicroscopyVital DyesCFSEKi67PCNABromo-deoxyuridine (BrdU)Chloro-deoxyuridine (CldU)Iodo-deoxyuridine (IdU)Ethinyl-deoxyuridine (EdU)Radio-detectionImmuno-detectionChemical DetectionAdult MiceImmunostaining ProtocolsCell Turnover

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