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Immunofluorescent Detection of Two Thymidine Analogues (CldU and IdU) in Primary Tissue
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Biologia
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JoVE Journal Biologia
Immunofluorescent Detection of Two Thymidine Analogues (CldU and IdU) in Primary Tissue

Immunofluorescent Detection of Two Thymidine Analogues (CldU and IdU) in Primary Tissue

DOI:
10.3791/2166-v

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10:55 min

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December 07, 2010

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, , , , , , , , , , ,
1Division of Endocrinology and Diabetes, Children’s Hospital of Philadelphia, Institute of Diabetes Obesity and Metabolism, Institute for Regenerative Medicine, Department of Pediatrics,University of Pennsylvania-School of Medicine

Capitoli

  • 00:01Titolo
  • 02:53Primary and Secondary Antibodies
  • 09:35Imaging and Analysis

Summary

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We have derived a strategy to detect sequential incorporation of thymidine analogues (CldU and IdU) into tissues of adult mice to quantify two successive rounds of cell division. This strategy is useful to detect cell turnover of long-lived tissues, oncogenic transformation, or transit-amplifying cells.

Tags

Immunofluorescent DetectionThymidine AnaloguesCldUIdUCell Division MeasurementExperimental BiologyMicroscopyVital DyesCFSEKi67PCNABromo-deoxyuridine (BrdU)Chloro-deoxyuridine (CldU)Iodo-deoxyuridine (IdU)Ethinyl-deoxyuridine (EdU)Radio-detectionImmuno-detectionChemical DetectionAdult MiceImmunostaining ProtocolsCell Turnover

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