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高度耦合的大鼠心肌线粒体的制备
JoVE Journal
Biologia
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JoVE Journal Biologia
Preparation of Highly Coupled Rat Heart Mitochondria
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高度耦合的大鼠心肌线粒体的制备

DOI:
10.3791/2202-v

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12:28 min

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September 23, 2010

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1Faculty of Life Sciences,University of Manchester, 2School of Biological Sciences,Queen’s University Belfast

Capitoli

  • 00:00Titolo
  • 00:07Introduction
  • 00:14General Scheme of the Protocol
  • 12:19Conclusion

Summary

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我们描述а纯，高度耦合的大鼠心脏细胞生物能，生物物理测量学，蛋白质组学或线粒体DNA和脂质分析的功能或结构的研究线粒体隔离协议。

Tags

Keywords: MitochondriaHighly CoupledRat HeartOxidative PhosphorylationEnergy GenerationApoptosisSignalling OrganellesMammalian DevelopmentAgeingCell MetabolismCell ProliferationBiological ProcessesIsolationHandlingIntact MitochondriaLaboratory AnimalsCellular MetabolismKnock-out AnimalsRapid MethodFunctional ResearchStructural ResearchBioenergeticsBiomolecule TransportProteomic StudiesMitochondrial DNAProteinsLipids

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