Encyclopedia of Experiments: Neuroscience
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Transcript
Begin with nerve stem cells cultured on an extracellular matrix-coated coverslip in a suitable differentiation medium.
Take a syringe containing a cold plasma, which is a state of matter containing various reactive components, including UV radiation and charged particles.
Position the nozzle of the plasma syringe at a specific height above the nerve cell culture.
Start the plasma jet.
The plasma interacts with the nerve stem cells, activating them.
Remove the old culture medium. Add a fresh culture medium and incubate.
Over time, the activated nerve stem cells produce projections.
Discard the medium.
Under an inverted phase contrast light microscope, the presence of elongated projections on the cells, resembling those found in a nerve cell, indicates differentiation.
