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Capitoli
- 00:05Titolo
- 02:23Performing the Capture Assay and Controls
- 08:26Tryptic Digest of Captured Proteins and Preparation of Peptides for LC-MS/MS
- 09:50Peptide and Protein Identification via Automated Sequence Database Search
- 12:33Representative Results of CCMS Experiments
- 16:00Conclusion
Yakalama Bileşikler fotoğraf crosslinking ve saflaştırma takip fonksiyonel geri dönüşümlü küçük molekül-protein etkileşim proteom karmaşıklığını azaltmak için trifunctional küçük moleküllerdir. Burada Yakalama Bileşik<em> S</em> Adenosil-<sub> L</subBir methyltransferases izole> seçicilik fonksiyonu olarak homosistein bağlayıcı<em> Escherichia coli</em> Tüm hücre lizat ve MS bunları tanımlamak.
Tags
MethyltransferasesS-adenosyl-L-homocysteine-binding ProteinsCapture Compound Mass SpectrometryAffinity ChromatographyActivity-based Protein ProfilingTrifunctional Capture CompoundsPhoto-crosslinkingSmall Molecule-protein InteractionsSelectivity FunctionSAHSorting FunctionBiotinSolid PhaseStreptavidin Magnetic BeadsOff-bead ConfigurationOn-bead ConfigurationSmall Molecule Of InterestSAMSAM-dependent MethyltransferasesDNA Methylation
