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Application of a NMDA Receptor Conductance in Rat Midbrain Dopaminergic Neurons Using the Dynamic Clamp Technique
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Neuroscienze
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JoVE Journal Neuroscienze
Application of a NMDA Receptor Conductance in Rat Midbrain Dopaminergic Neurons Using the Dynamic Clamp Technique

Application of a NMDA Receptor Conductance in Rat Midbrain Dopaminergic Neurons Using the Dynamic Clamp Technique

DOI:
10.3791/2275-v

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06:42 min

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December 21, 2010

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,
1Neurosciences Institute,University of Texas San Antonio – UTSA

Capitoli

  • 00:05Titolo
  • 00:58Introduction
  • 01:17Slice Preparation
  • 01:44Electrophysiological Recording
  • 04:09Conductance Application with Dynamic Clamp
  • 05:37Representative Recording Results
  • 06:02Conclusion

Summary

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In this video, we demonstrate how to apply a conductance into a dopaminergic neuron recorded in the whole cell configuration in rat brain slices. This technique is called the dynamic clamp.

Tags

NMDA Receptor ConductanceRat Midbrain Dopaminergic NeuronsDynamic Clamp TechniqueNeuroscientistsBrain CommunicationElectrical ActivityPatch Clamp TechniquesGlass MicropipettesDirect Current InjectionIon ChannelsNeuronal MembraneRectangular PulsesKinetics Of Ion ChannelsChemical Changes In The CellReceptor ActivationElectrical StimulationPharmacological Stimulation

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