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蠕虫病毒实验室介绍：从培养蠕虫突变
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Biologia
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JoVE Journal Biologia
An Introduction to Worm Lab: from Culturing Worms to Mutagenesis
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蠕虫病毒实验室介绍：从培养蠕虫突变

DOI:
10.3791/2293-v

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10:44 min

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January 11, 2011

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, ,
1Department of Biology,University of Texas at Arlington

Capitoli

  • 00:05Titolo
  • 00:57Introduction
  • 01:41Making a Pick
  • 02:45Picking Worms
  • 03:40Ethyl Methane Sulfonate (EMS) Mutagenesis
  • 06:33Psoralen Mutagenesis
  • 09:27Results of Mutagenesis of P. pacificus
  • 10:01Conclusion

Summary

Traduzione automatica

Traduzione automatica

与表型的缺陷突变体的筛选是一个简单的方法识别基因功能，在一个给定的生物过程。在这篇文章中我们将介绍如何对文化自由的生活的蠕虫（如，<em> Pristionchus pacificus</em>）在实验室中，并显示两个不同的诱变方法，环境管理体系和TMP /紫外线。

Tags

Worm LabCulturing WormsMutagenesisNematodesLaboratory MaintenanceEthyl Methane SulfonateEMS45′8-trimethylpsoralenTMP/UVGenetics StudiesSelf-fertilizing HermaphroditesMating SystemAgar PlatesBacterial LawnPoint MutationsSmall DeletionsRecessive MutationsPristionchus PacificusPhenotypesUncoordinated MutantsDumpy MutantsTransformer Mutants

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